The univariate analysis revealed that poor survival was significantly correlated with high αSMA expression (p<0.001), high THBS4 expression (p<0.001), age ≥ 65 years old (p<0.001), macroscopic type 4 (p<0.001), microscopic undifferentiated type (p<0.001), high T stage (p<0.001), lymph node metastasis (p<0.001), lymphatic invasion (p<0.001), vascular invasion (p<0.001), ascites cytology positive (p<0.001), peritoneal metastasis (p<0.001), and hepatic metastasis (p<0.001). Here, ACTA1 is linked to metastatic malignant neoplasm in the lymph nodes.