For the exploration of the biological role of CERS6‐AS1 in the development of BC, we upregulated CERS6‐AS1 through utilizing pcDNA‐CERS6‐AS1 with vector as scramble control and knocked down CERS6‐AS1 through using shCERS6‐AS1#1, shCERS6‐AS1#2, shCERS6‐AS1#3 with shCtrl as scramble control. This evidence concerns the gene CERS6 and breast cancer.