CD8A and cancer: A high ratio of CD8/CD4, CD25 cells is predictive of lower lymph nodes metastasis and overall increased survival in cancer patients.45 No alterations in the percentage of total T cells (CD45+, CD3+), CD4 (CD45+, CD3+, CD4+), or CD8 (CD45+, CD3+, CD8+) T cells were observed in the tumors (Fig. 4a) or in the spleen and intact mammary glands (Supplementary Fig. 6).