TWIST2 and metabolic disease: Therefore, LT strongly inhibited the expression of tapetum development genes, such as TDF1, EAT1, and AMS, in 05ms and blocked the expression of the downstream gene 4CLL1. Additionally, a secondary metabolic disorder led to abnormal development of the middle cell layer and tapetum of the anther cell wall in eggplant rTGMS 05ms, which caused male sterility.