Therefore, LT strongly inhibited the expression of tapetum development genes, such as TDF1, EAT1, and AMS, in 05ms and blocked the expression of the downstream gene 4CLL1. Additionally, a secondary metabolic disorder led to abnormal development of the middle cell layer and tapetum of the anther cell wall in eggplant rTGMS 05ms, which caused male sterility. The gene discussed is TWIST2; the disease is Other metabolic disease.