For example, the eight PDCs harbored the single-nucleotide variant (SNV)/indel overlapping genes found in the 161 ESCC patients (recurrence ≥5%), including AR, ARID1A, CDKN2A, EP300, KMT2D, LRP1B, NF1, NOTCH1, SPTA1, and TP53 (Supplementary Fig. 4a). This evidence concerns the gene NOTCH1 and esophageal squamous cell carcinoma.