We found that mRNA expression of SREBP1c, FAS, consistent with the corresponding protein level (Fig. 4d), and ACC1 was significantly upregulated (p < 0.001) in NASH-induced liver compared to CTL group, while SREBP1c-mediated DNL pathway was considerably inhibited (p < 0.001 for all genes) in NASH + ISO group when compared with NASH group (Fig. 4c). The gene discussed is ACACA; the disease is metabolic dysfunction-associated steatohepatitis.