To examine AML cell death in response to FTY720, MV4-11 cells were treated with a dose response of FTY720 and plasma membrane asymmetry and integrity were examined by staining with fluorophore-conjugated Annexin V (Ann V) and the cell-impermeable nucleic acid binding dye 7-AAD. The gene discussed is ANXA5; the disease is acute myeloid leukemia.