After the knockdown of PVT1 in BMSCs, the BMSC-EXOsi-PVT1 which contains lower amounts of PVT1 than normal BMSC-EXO was obtained, and the effect of BMSC-EXOsi-PVT1 on osteosarcoma metastasis was inhibited, suggesting that the knockdown of PVT1 in BMSCs exerts the tumor-suppressing effect and may become a novel therapeutic strategy of osteosarcoma. The gene discussed is PVT1; the disease is osteosarcoma.