Mice injected with NOTCH2KO CNE-2 cells developed significantly more metastatic foci in the liver than mice injected with Ctrl CNE-2 cells with no altered primary tumor growth in the spleen (P < 0.05; Fig. 2e), indicating that NOTCH2 suppression promotes NPC cell metastasis in vivo. Here, NOTCH2 is linked to nasopharyngeal carcinoma.