However, Western blotting showed that several members of the MAPK family, including ERK1/2, JNK1/2 and P38, were unaffected after knockdown of NOTCH2 in CNE-2 cells (P > 0.05; Fig. 5b), indicating that MAPK signaling is not involved in the NOTCH2-mediated regulation of EMT in NPC. This evidence concerns the gene NOTCH2 and nasopharyngeal carcinoma.