For example, co-expression of IL-2 and tumor necrosis factor (TNF-α), linked by IRES, in an oncolytic adenovirus genome, has allowed increases in the number of CD4+ and CD8+ tumor-infiltrating lymphocytes, as well as T-helper 1 (Th1) and CD86+ dendritic cells (DCs) in vivo [90,92]. The gene discussed is CD8A; the disease is neoplasm.