HCC cells transfected with mock control, inhibitor negative control, miR-221 mimic, miR-221 inhibitor, and AEG-1 siRNA and cell cycle analysis was performed using PI staining and apoptosis measured using Annexin V-FITC/PI duel-staining with Alexa Fluor-conjugation. The gene discussed is ANXA5; the disease is hepatocellular carcinoma.