The regulative mRNA expressions of angiogenesis (LSF and MMP9), anti-apoptotic (OPN, and Bcl2), autophagy (LC3A) and PI3K/Akt were decreased and cell cycle regulatory mRNA expressions (PTEN, p57, p53, and RB) were increased (Figure 2 and Figure 3) in miR-221 inhibitor and AEG-1 siRNA transfected HCC cells. This evidence concerns the gene PTEN and hepatocellular carcinoma.