MTDH and hepatocellular carcinoma: To investigate the role of AEG-1 and miR-221 in cell migration, invasion, and angiogenesis we used wound healing, transwell, and tube formation assay performed in miR-221 mimic, miR-221 inhibitor, AEG-1 siRNA and their controls transfected HCC and HUVECs cells in in vitro.