F11R and infection: To test the notion that K134 and R234 are the key residues for JAM1 degradation, we performed immunofluorescence assays in IHGE cells expressing HA-tagged JAM1 Δ (1–133) or HA-tagged JAM1 Δ (1–133) K134H R234H following infection with P. gingivalis. One hour after infection, in IHGE cells expressing HA-tagged JAM1 Δ (1–133) the anti-HA signal was decreased by P. gingivalis WT, but not by the Δkgp ΔrgpA ΔrgpB mutant (Fig 5C).