Figure 5B showed MFHAS1 overexpression decreased the expression levels of TNF-α, IL-1β or IL-6. It indicated that MFHAS1 could inhibited inflammation in vivo. In addition, TGF-β, fibronectin and collagen IV expressions were obviously down-regulated in STZ+MFHAS1 group when compared with other groups (P<0.001) (Figure 5C,D) in STZ mice. These results demonstrated that overexpression of MFHAS1 effectively relieved the symptoms of DN in vivo experiment. This evidence concerns the gene TGFB1 and liver dysplastic nodule.