TNF and liver dysplastic nodule: Figure 5B showed MFHAS1 overexpression decreased the expression levels of TNF-α, IL-1β or IL-6. It indicated that MFHAS1 could inhibited inflammation in vivo. In addition, TGF-β, fibronectin and collagen IV expressions were obviously down-regulated in STZ+MFHAS1 group when compared with other groups (P<0.001) (Figure 5C,D) in STZ mice. These results demonstrated that overexpression of MFHAS1 effectively relieved the symptoms of DN in vivo experiment.