Immunofluorescence assay confirmed that the gastric cancer cells, which HOXA11 was induced, have experienced the epithelial-mesenchymal transition, the cells showed less differentiated morphology, which was characterized by tight epithelial cobblestone islands (Figure 2A), decreased expression of markers of epithelial differentiation (E-cadherin) and increased mesenchymal markers (Fibronectin, N-cadherin, Twist1 and α-SMA) and vice versa (Figure 2B and Figure S1G). This evidence concerns the gene TWIST1 and gastric cancer.