We then investigated the changes of expression profiles in the hepatocellular carcinoma cell line HepG2 in the setting of overexpression of MEG3. When compared with the vector controls, 68 DE miRNAs (15 up- and 53 down-regulated) and 50 DE mRNAs (25 up- and 25 down-regulated) were identified in the HepG2 cell line (Figs 5b,c and S7b,c), of which 21 miRNAs were potentially interacted with MEG3 (e.g., miR-195-5p). Here, MEG3 is linked to hepatocellular carcinoma.