NES and non-small cell lung carcinoma: To test whether Nrf2 regulated Nestin transcription by binding to the AREs located in the Nestin promoter, NSCLC cells were treated with the Nrf2 activator, tBHQ, we found that the luciferase activity were enhanced by hARE1 and hARE2, rather than hARE3 of the Nestin promoter (Fig. 6b).