To determine whether NS3/4A controls invadopodium precursor assembly and activation via the EGFR signaling pathway, we repeated the invadopodium precursor assembly and matrix degradation experiments by ectopically expressing the NS3/4A protease in HCC cells in the presence of the EGFR kinase inhibitor erlotinib. This evidence concerns the gene EGFR and hepatocellular carcinoma.