PIWIL1 and colorectal carcinoma: Interestingly, we observed in human CRC cells that PIWIL1 localizes in nuage-like perinuclear granules in the cytoplasmic perinuclear region of the cell, a result that, combined with the identification of mature piRNAs carrying a methyl group at their 3′-end, indicates the presence of an active piRNA pathway in CRC cells.