In the clinical practice, nowadays, a few methylation markers are validated, such as septin 9 (SEPT9) in colorectal cancer [51], and several groups have reported that the analysis of circulating methylated tumor suppressor genes could be used for the non-invasive detection of human tumors, including HCC [43,52,53,54,55]. This evidence concerns the gene SEPTIN9 and hepatocellular carcinoma.