To explore the underlying mechanism of ROR1-AS1 function in CRC, RT-qPCR and Western blot assay were conducted to detect the target proteins in Wnt/β-catenin signaling pathway such as Wnt3a, β-catenin, C-myc and Survivin in SW620 CRC cells. The gene discussed is BIRC5; the disease is colorectal carcinoma.