Xenograft tumor in nude mice was performed to evaluate tumorigenesis, which showed that compared with the NC-mimic group and the si-NC group respectively, mice in the miR-143-3p mimic group and si-MSI2 group presented remarkably attenuated tumor growth rate (all P<0.05), while the growth rate of subcutaneous tumors in the miR-143-3p inhibitor + si-MSI2 group was similar to that in the control group (P>0.05; Figure 8A–C). This evidence concerns the gene MSI2 and neoplasm.