To investigate the involvement of UBA6 in autophagy, we initially used siRNAs to KD UBA6 in H4 and HeLa cervical carcinoma cells, and examined the levels of the cytosolic (LC3B-I) and membrane-bound (LC3B-II) forms of LC3B as indicators of autophagy status (Kabeya et al., 2004) (Figure 2C and D). The gene discussed is MAP1LC3B; the disease is cervical carcinoma.