To research the biological roles of YAP1 in SCLC, we developed H69 stable cell lines that overexpress constitutively active YAP1 and H446 stable cell lines that dominate negative YAP1, while we used H69‐NC and H446‐NC as negative controls.28 We then used Western blot to verify the transfection (Figure 2C,D). The gene discussed is YAP1; the disease is small cell lung carcinoma.