Many somatic mutations were found in the tumor tissues of this patient with prolactinoma, including Dmx like 2 (DMXL2), cytochrome P450 family 4 subfamily B member 1 (CYP4B1), Epidermal growth factor (EGF), mutY DNA glycosylase (MUTYH), radial spoke head 6 homolog A (RSPH6A), solute carrier organic anion transporter family member 1B3 (SLCO1B3), and POU6F2. DMXL2 and POU6F2 were confirmed by sanger sequencing. The gene discussed is POU6F2; the disease is neoplasm.