To better clarify the delayed bone healing in T2DM rats, we measured the expression of osteoblast‐related genes including alkaline phosphatase (ALP), osteocalcin (OCN), runt‐related transcription factor 2 (RUNX2) and collagen a1 (COLIA1) in newly generated tissue at the fracture site. Here, RUNX2 is linked to type 2 diabetes mellitus.