Using the median level of RNA expression as the cut-off point, Kaplan–Meier survival analysis showed that high levels of lncNB1, DEPDC1B, RPL35, and E2F1 RNA expression in the 493 neuroblastoma tissues of the SEQC-RPM-seqcnb1 dataset were associated with poor patient prognosis (Fig. 8e–h). This evidence concerns the gene E2F1 and neuroblastoma.