Alamar blue assays showed that knocking down lncNB1 expression reduced the numbers of viable cells (Fig. 6a), BrdU incorporation assays showed that lncNB1 knockdown consistently reduced neuroblastoma cell proliferation (Supplementary Fig. 8), and flow cytometry analysis of Annexin V positively stained cells showed that knocking down lncNB1 induced apoptosis (Fig. 6b), suggesting that lncNB1 is required for neuroblastoma cell proliferation and survival. This evidence concerns the gene ANXA5 and neuroblastoma.