In addition, Affymetrix microarray experiments revealed that lncNB1 knockdown resulted in reduction in the expression of 34 mRNAs, including E2F1 mRNA, in the heavy polysomes (Supplementary Table 5), and fluorescence in situ hybridization and immunocytochemistry double labeling experiments showed that lncNB1 RNA and RPL35 protein were mostly located in the cytoplasm in neuroblastoma cells (Supplementary Fig. 7a, b). Here, E2F1 is linked to neuroblastoma.