Knockdown of E2F1 expression with two independent siRNAs reduced DEPDC1B mRNA and protein expression in both BE(2)-C and Kelly neuroblastoma cell lines (Fig. 4b, c), and replicated the effects of lncNB1 siRNAs, lncNB1 shRNAs, and DEPDC1B siRNAs in reducing ERK protein phosphorylation, N-Myc protein phosphorylation at S62 and N-Myc protein expression in BE(2)-C and Kelly cells (Supplementary Fig. 5c). Here, MYCN is linked to neuroblastoma.