MYCN and neuroblastoma: Consistent with these data, transfection with DEPDC1B siRNAs reduced DEPDC1B but not N-Myc mRNA expression (Supplementary Fig. 3b, c), and reduced DEPDC1B protein expression, ERK protein phosphorylation, N-Myc protein phosphorylation at S62 and expression in the three neuroblastoma cell lines (Fig. 3b).