RT-PCR and immunoblot analyses confirmed that lncNB1 siRNAs consistently reduced DEPDC1B mRNA and protein as well as E2F1 protein, but did not show a consistent effect on E2F1 mRNA, in BE(2)-C, Kelly and CHP134 neuroblastoma cells (Fig. 2b, c). This evidence concerns the gene E2F1 and neuroblastoma.