To investigate whether chronic TCR-A2 (self)-interactions could lead to the previously observed hyporesponsive state (Figs. 4 and 5), we performed extended co-cultures between A2neg primary CD8 T cells and either A2pos or A2neg NA8 cells, by passing the T cells over fresh layers of tumor cells every 3–4 days (Fig. 7 a). The gene discussed is CD8A; the disease is neoplasm.