In the current study, we found that melanoma cells augmented the expression of various mesenchymal phenotypic markers, such as Smad2/3, α-SMA, and vimentin, in addition to that of TGF-β, Snail, and Slug, following exposure to Tregs (Figure 3A,B and Figure 4B,C). This evidence concerns the gene SMAD2 and melanoma.