KLHDC4 and breast carcinoma: CRISPR-Cas9 mediated knockout of particular genes such as SHCBP1 (SH2-domain binding protein 1) in breast cancer cells in vitro [70] and KLHDC4 (kelch domain containing 4) in nasopharyngeal carcinoma cells, both in vitro and in vivo [71], has shown to generate positive outcomes, with both knockouts associated with reduced proliferation of cells as well as inhibited cell migration and invasion in the latter.