Of the 358 genes, a total of 15 genes (SOX2, KIT, PRSS56, WNT9A, SLC4A4, PRRG4, PANX2, PIR, RASSF8, MFSD4A, INS, RNF39, IL1R2, CST1, and PPP3CA) demonstrated significant association with the prognosis of CCA (Table 3). Here, PPP3CA is linked to cholangiocarcinoma.