To establish iPSCs for SCA3, primary dermal fibroblasts from two SCA3 patients (SCA3-1: 47-year-old female; SCA3-2: 50-year-old male) and two normal controls (NC-1: 63-year-old female; NC-2: 31-year-old male) were transfected with four reprogramming factors OCT4, KLF4, SOX2, and c-MYC by retroviruses. The gene discussed is SOX2; the disease is Machado-Joseph disease.