In addition, the decline in NRF1 and NRF2 mRNA levels from benign naevi to melanomas could be reproduced when comparing the NRF1 and NRF2 mRNA levels in cell culture lysates in the qPCR, showing a decrease of relative mRNA levels between benign melanocyte and malignant melanoma cells (Figure 1(b)). Here, NRF1 is linked to melanoma.