In particular, before any eventual therapeutic relevance, it is required to overcome two important limitations of this study: (i) while supported by in vitro observations, the role of COL3A1 in cancer cell spreading should be extended on animal models of cholangiocarcinoma [46]; (ii) type III collagen alignment should be integrated and interpreted in a larger cohort of iCCA patients at different stages of the disease. The gene discussed is COL3A1; the disease is infantile convulsions and choreoathetosis.