In diabetes, after the combination of insulin and growth factor receptor tyrosine kinases, PI3K is activated and generates phosphatidylinositol 3,4,5-trisphosphate, which induces the phosphorylation of AKT (p-AKT); p-AKT then activates endothelial nitric oxide synthase (eNOS) and produces NO. This evidence concerns the gene NOS3 and diabetes mellitus.