Thus, we next chose DU145 and 22RV1 cells to generate DEPTOR depleted cells via a CRISPR/Cas9-based approach and found that DEPTOR depletion significantly promoted cell proliferation in two individual colonies of sgDEPTOR cells in both prostate cancer cell lines, compared with that in sgCtrl cells (Fig. 2a). The gene discussed is DEPTOR; the disease is prostate carcinoma.