We used ACTB protein as an internal control for salivary protein analysis based on published reports.26,27 This abundant cytoskeletal protein is used as an indicator of saliva quality for proteomic analyses and is a good biomarker of oral squamous cell carcinoma (OSCC).28 The concentrations of ACTB are significantly higher in patients with pre-OSCC than those in healthy individuals. This evidence concerns the gene ACTB and oral cavity squamous cell carcinoma.