CD274 and non-small cell lung carcinoma: In order to assess the predictive value of TIS score within the context of one of other biomarkers of clinical interest, we also scored the NSCLC samples for PD-L1 expression by IHC at the 1% and 50% cutoff for tumor cell positivity, the two cut offs reported in the label for second and first line NSCLC patient selection for treatment with pembrolizumab.