Another liver tissue sample was subjected to two-step qRT-PCR for examination of changes in mRNA expression of oxidative stress and inflammation markers and TG synthesis regulators, such as TNF-a, IL-6, GPAT-1, ACC and SREBP-1c, which were associated closely with NAFLD (Fig. 2). This evidence concerns the gene SREBF1 and metabolic dysfunction-associated steatotic liver disease.