ΔΔCt values in multiple and single infections were respectively 3.26 (95% confidence interval 2.60–3.92) and 3.98 (95% confidence interval 3.61–4.35) for TOP2A (p = 0.044); 3.22 (95% confidence interval 2.66–3.79) and 4.12 (95% confidence interval 3.73–4.50) for MKI67 (p = 0.010) and 0.95 (95% confidence interval 0.44–1.46) vs. 1.84 (95% confidence interval CI 1.52–2.16) for CDKN2A/p16 (p = 0.003). The gene discussed is TOP2A; the disease is infection.