NOS3 and Hyperglycemia: Additionally, senescence can trigger stimuli, such as reactive oxygen species [2], hyperglycemia [3], inflammatory cytokines [4], and telomere dysfunction [5], and is promoted under high glucose (HG) conditions in vitro [6], which can cause cellular injury by induction of oxidative stress [7], apoptosis [8], and downregulation of endothelial nitric oxide synthase (eNOS) [9].