However, n-butylidenephthalide (BP), which is isolated from the chloroform triggered both p53-dependent and -independent pathways for apoptosis in vitro on glioblastoma multiforme (GBM) cells and suppressed growth of subcutaneous rat and human brain tumors and also, reduced the volume of GBM tumors in vivo [212]. The gene discussed is TP53; the disease is glioblastoma.