HSP90B1 and colorectal carcinoma: To investigate the binding of GRP94 IgG to endogenous GRP94 on CRC cells, we performed flow cytometry and found that GRP94 IgG binds strongly to endogenous GRP94 on the surfaces of five CRC cell lines, including HCT116, HT29, LoVo, HCT-8, and Caco-2 cells (Figure 2A).