In the C-vs-L comparison, the most enriched pathway was “Non-alcoholic fatty liver disease (NAFLD)”, followed by the longevity-regulating pathway—worm, pentose phosphate pathway, Toll and Imd signaling pathways, insect hormone biosynthesis and tyrosine metabolism (Figure 7A). The gene discussed is TLR4; the disease is metabolic dysfunction-associated steatotic liver disease.