Fourteen days after i.v. tumour inoculation, disseminated tumour growth induced an increase of Tregs (Foxp3+CD25+ cells gated on CD4+) in the spleen from 6.8 ± 1.7% in naïve mice to 18.8 ± 3.6% in tumour-bearing mice (Figure 6A,B). This evidence concerns the gene CD4 and neoplasm.