VGF and Familial prostate cancer: Finally, we also considered the prostate cancer cell line PC3 and could confirm the efficiency of VGF knockdowns and we also observed a moderately increased radiosensitivity in response to VGF knockdowns (S9 Fig, e.g. t-test: siRNA VGF #2 vs. negative control: P < 0.03 at 4 Gy, S7 Table).