IFI16 and osteosarcoma: To further confirm these results, we used IFI16 CRISPR knockout (KO) osteosarcoma U2OS cells (U2OS 67) generated in our earlier studies (Johnson et al., 2014) to study the effect of IFI16 KO and re-introduction of IFI16 in a KO background on the recruitment of H3K9me3, SUV39H1 and GLP.