Upregulated (Huang et al., 2009) MiR-16 targets and regulates Bcl-2 and suppresses Bcl-2 protein expression by binding to the 3′UTR of Bcl-2 mRNA. Bcl-2 is one of the well-known anti apoptotic protein members in Bcl-2 family that control cancer cells response to drugs (Wang et al., 2018). RHepG2 cells, the multidrug resistant subgroup of human HCC HepG2 cells were reported to have higher levels of miR-16 and P-glycoprotein which is associated with multidrug resistance and lower level of Bcl-2 (Fregni et al., 2018). Here, BCL2 is linked to hepatocellular carcinoma.