Secondly, since the real hub genes belong to the brown module, which was significantly related to the histological grade and pathological stage of BC, the expression pattern of ANLN (Figure 6) and HMMR (Figure 7) in different histological grade and pathological stage were verified in internal validation datasets (GSE71576) and external validation datasets (GSE13507, GSE31684, and TCGA dataset). The gene discussed is ANLN; the disease is breast cancer.