Two weeks after injection of DHM or 1 week after injection of P2X7 shRNA, the MWT and TWL were significantly lower in the model + P2X7 shRNA and model + DHM groups than in the model group (p < 0.05), indicating that both P2X7 shRNA and DHM treatment relieve neuropathic pain–related behavior in rats with DNP and MDD (Figures 2A, B). Here, P2RX7 is linked to major depressive disorder.