Ex vivoCD40L-EVs were more immunogenic as compared with unmodified EVs:CD40L-EVs induced ↑ mature DC phenotype and ↑ levels of IL-12 and TNF-α synthesis by DCs.DC/CD40L-EVs induced ↑ proliferation of allogeneic T cells.CD40L-EVs induced ↑ proliferation of tumour antigen-specific CD4+ T cells.In vivo3LL Lewis lung carcinoma modelRetardation of tumour growth; overall survival:A. Prophylactic vaccination (SC).CD40L-EVs – in 16 times; 80%.EVs – in 3 times; 50%.B. Therapeutic vaccination (SC).CD40L-EVs – in 10 times; 50%.EVs – in 1.7 times; 0%. This evidence concerns the gene TNF and neoplasm.